Archives for December, 2016

Rapid Plasma Reagin (RPR) test: Principle, Procedure, Result and Interpretation

INTRODUCTION : Rapid plasma reagin is commonly abbreviated as RPR. RPR test is a macroscopic, nontreponemal, flocculation test. It is a non-specific screening test for diagnosis of syphilis. Syphilis is a venereal disease caused by the spirochete Treponema pallidum.  After acquiring infection, the host forms antibodies to Treponema pallidum, in addition, the host also forms […]

Diene’s stain for identification of Mycoplasmas

Mycoplasma colonies can be identified by observing typical fried egg colonies on culture medium. Visualization is facilitated by application of Diene’s stain directly to the agar surface. Diene’s stain is a nonspecific stain that imparts a contrasting appearance to Mycoplasma colonies on agar. It allows easy visualization of colony morphology and characteristics. Composition of Diene’s […]