Archives for Staining techniques
Albert Stain: Principle, Procedure and Observation
Principle: Albert stain is done to identify Corynebacterium diphtheriae. This stain differentially stains the metachromatic granules and cytoplasm of this bacterium. Procedure: Prepare a smear and fix it by gently…
Ziehl Neelsen Staining or AFB Staining Procedure
Smear preparation for AFB: Take a grease free scratch free glass slide to prepare the smear. (A new glass slide should be used for smear preparation, if greasy; you can…
Gram Staining Procedure
First of all, prepare a smear and let it air dry. Fix the smear with heat rolling the slide over blue flame for three times. Pour crystal violet over the…