Archives for April, 2014

Voges Proskauer test (VP test): Principle, Procedure, Interpretation and Quality Control

Voges-Proskauer test is one of the tests used for identification of Enterobacteriaceae. It is usually performed alongside the methyl red test since both tests are performed on cultures grown in MR-VP broth.  Both tests are based on the detection of end products from the metabolism of glucose. VP test is based on the detection of […]

Oxidative/Fermentative Test (O/F test)

Oxidative/Fermentative (O/F) test is done to distinguish between aerobes and anaerobes through their ability to breakdown carbohydrates. In this test, a semi solid medium containing a carbohydrate (usually glucose) and a pH indicator is used.  If acid is produced only at the surface of the medium, where conditions are aerobic, the attack on sugar is […]