CAMP (Christie, Atkins, and Munch-Peterson) test is used for the presumptive identification of Group B Streptococcus (Streptococcus agalactiae). It is the only beta-hemolytic Streptococcus which yields a positive CAMP test. The test has been named after Christie, Atkins, and Munch-Peterson, who described it in 1944. a Principle of CAMP test CAMP test is done to detect […]
Principle of Leucine amino peptidase (LAP) test: Leucine amino peptidase (LAP) test is used for the preliminary characterization of catalase negative, gram-positive cocci. This is a rapid test for detection of enzyme leucine aminopeptidase. Leucine- β- napthalamide impregnated disk serves as a substrate for the detection of leucine aminopeptidas. This enzyme hydrolyse the substrate resulting […]
Gelatin hydrolysis is helpful in identifying and differentiating species of Bacillus, Clostridium, Proteus, Pseudomonas, and Serratia. It also distinguishes the gelatinase-positive, pathogenic Staphylococcus aureus from the gelatinase-negative, non-pathogenic S. epidermidis . Gram-positive, spore-forming, rodshaped, aerobic or anaerobic bacteria such as Bacillus anthracis, Bacillus cereus, Bacillus subtilis, Clostridium perfringens and Clostridium tetani, are also positive for […]
Hippurate hydrolysis test can be used for presumptive identification of Gardnerella vaginalis, Campylobacter jejuni, Listeria monocytogenes and group B streptococci. Principle of Hippurate hydrolysis test Hippurate hydrolysis test is used to detect the ability of bacteria to hydrolyse hippurate into glycine and benzoic acid by action of hippuricase enzyme present in bacteria. Previously, it was tested using ferric chloride […]