Author Archives: Prasil Pradhan - Page 2
Oxidative/Fermentative Test (O/F test)
Oxidative/Fermentative (O/F) test is done to distinguish between aerobes and anaerobes through their ability to breakdown carbohydrates. In this test, a semi solid medium containing a carbohydrate (usually glucose) and…
Novobiocin susceptibility test
The speciation of coagulase negative Staphylococcus is necessary in certain culture specimens. Coagulase negative staphylococci can either be resistant or susceptible to novobiocin. The one most commonly isolated novobiocin resistant…
Life Cycle of Round worm (Ascaris lumbricoides)
Roundworm passes its life cycle in single host and no intermediate host (the host that harbors larval stage of parasite) is required. Man is the only definitive host (the host…
Albert Stain: Principle, Procedure and Observation
Principle: Albert stain is done to identify Corynebacterium diphtheriae. This stain differentially stains the metachromatic granules and cytoplasm of this bacterium. Procedure: Prepare a smear and fix it by gently…
Bile solubility test: Principle, Procedure and Expected results
Principle of Bile Solubility test: Bile solubility test is used to differentiate Streptococcus pneumoniae from other alpha-hemolytic Streptococci. Streptococcus pneumoniae is bile soluble whereas all other alpha-hemolytic streptococci are insoluble. Bile…
Optochin Sensitivity Test: Principle, Procedure and Interpretation
Principle: Optochin (ethyl hydrocupreine hydrochloride) sensitivity test is used for the presumptive identification of alpha-hemolytic streptococci as Streptococcus pneumoniae. Optochin is used to differentiate Streptococcus pneumoniae from other alpha-hemolytic streptococci.…
CAMP test and Reverse CAMP test
CAMP (Christie, Atkins, and Munch-Peterson) test is used for the presumptive identification of Group B Streptococcus (Streptococcus agalactiae). It is the only beta-hemolytic Streptococcus which yields a positive CAMP test. The…
Leucine amino peptidase (LAP) test for identification of catalase negative gram positive cocci
Principle of Leucine amino peptidase (LAP) test: Leucine amino peptidase (LAP) test is used for the preliminary characterization of catalase negative, gram-positive cocci. This is a rapid test for detection…
Gelatin hydrolysis test: Principle, Procedure, Interpretation and preparation of nutrient gelatin medium
Gelatin hydrolysis is helpful in identifying and differentiating species of Bacillus, Clostridium, Proteus, Pseudomonas, and Serratia. It also distinguishes the gelatinase-positive, pathogenic Staphylococcus aureus from the gelatinase-negative, non-pathogenic S. epidermidis…
Hippurate hydrolysis test
Hippurate hydrolysis test can be used for presumptive identification of Gardnerella vaginalis, Campylobacter jejuni, Listeria monocytogenes and group B streptococci. Principle of Hippurate hydrolysis test Hippurate hydrolysis test is used to detect the ability…