Superoxol test is a rapid test that can be used for presumptive identification of Neisseria gonorrhoea. It is similar to Catalase test in which 3% hydrogen peroxide (H2O2) solution is used but 30% hydrogen peroxide is used in this test. N. gonorrhoeae strains produce immediate, vigorous bubbling when the colony is emulsified with 30% H2O2. Both N. meningitidis and N. lactamica, and other species that grow on selective media, generally produce weak, delayed bubbling, although some isolates of N. meningitidis may also produce immediate, vigorous bubbling similar to the gonococcus in this test.
Organisms that are oxidase-positive, gram-negative diplococci recovered from urogenital sites and that grow on selective media may be presumptively identified as N. gonorrhoeae. Superoxol test provides an additional presumptive test for identifying these isolates.
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