Principle: Albert stain is done to identify Corynebacterium diphtheriae. This stain differentially stains the metachromatic granules and cytoplasm of this bacterium. Procedure: Prepare a smear and fix it by gently heat over a flame. Cover the smear with Albert stain (Albert I) staining solution. Allow it for 5 minutes. Pour off the stain after 5 […]

Smear preparation for AFB: Take a grease free scratch free glass slide to prepare the smear. (A new glass slide should be used for smear preparation, if greasy; you can clean the grease by wiping it with alcohol and use it after its air dry). Label the slide with laboratory code and serial number with […]

First of all, prepare a smear and let it air dry. Fix the smear with heat rolling the slide over blue flame for three times. Pour crystal violet over the smear and wait for 1 minute. Drain off excess stain and wash the stain with distilled water. Pour iodine over the stained smear and wait […]