Author Archives: Prasil Pradhan - Page 2

D test- A test for detection of inducible Clindamycin resistance in Staphylococcus aureus

D-test  detects inducible clindamycin resistance in Staphylococcus aureus by double disk diffusion using Clindamycin(2ug) and Erythromycin (15 ug) disks. Erythromycin is a macrolide and clindamycin is a lincosamide, two distinct classes of…
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ONPG (o-Nitrophenyl-β-D-Galactopyranoside) test: Principle, Procedure, Result and Interpretation

Principle: o-Nitrophenyl-β-D-Galactopyranoside (ONPG) is structurally similar to lactose except that o-nitrophenol has been substituted for glucose. On hydrolysis through the action of enzyme β-galactosidase, ONPG which is a colorless compound…
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Acetamide Utilization Test: Principle, Procedure, Expected results, Quality Control and Limitation

Acetamide Utilization test: This test is useful in differentiation of nonfermentative gram-negative bacteria, particularly Pseudomonas aeruginosa. Principle of Acetamide Utilization Test Acetamide utilization test is used to check the ability of…
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