Gram stain of sputum is a valuable test for the requesting physician. It helps the physician to judge whether the patient he/she has counseled to obtain the sputum has really submitted the sputum sample or merely an oropharyngeal secretion i.e. saliva. Generally, while preparing the sputum smear, purulent part of the sputum is taken and […]
Cystine Lactose Electrolyte Deficient (CLED) agar is a type of differential medium generally used for isolation and enumeration of bacteria from urine specimen form patients suspected of urinary tract infection. CLED agar supports the growth of all potential uropathogens such as gram negative bacilli belonging to Enterobacteriaceae and gram positive cocci such as Enterococcus spp, […]
Principle Sodium polyanethol sulfonate (SPS) is a commonly used anticoagulant. It is also known as Liquoid. It can inhibit the growth of certain anaerobic bacteria such as Peptostreptococcus anaerobius as well as aerobic Gardnerella vaginalis. So, this property of SPS can be used as a tool for differentiating P. anaerobius from other anaerobic cocci. For […]