Archives for December, 2012

Ziehl Neelsen Staining or AFB Staining Procedure

Smear preparation for AFB: Take a grease free scratch free glass slide to prepare the smear. (A new glass slide should be used for smear preparation, if greasy; you can clean the grease by wiping it with alcohol and use it after its air dry). Label the slide with laboratory code and serial number with […]

Gram Staining Procedure

First of all, prepare a smear and let it air dry. Fix the smear with heat rolling the slide over blue flame for three times. Pour crystal violet over the smear and wait for 1 minute. Drain off excess stain and wash the stain with distilled water. Pour iodine over the stained smear and wait […]